By Natan T. Shaked, Zeev Zalevsky, Lisa L Satterwhite
Written through best optical part microscopy specialists, this booklet is a accomplished connection with section microscopy and nanoscopy strategies for biomedical functions, together with differential interference distinction (DIC) microscopy, part distinction microscopy, electronic holographic microscopy, optical coherence tomography, tomographic section microscopy, spectral-domain section detection, and nanoparticle utilization for part nanoscopy
The Editors convey biomedical and optical engineers how you can use part microscopy for visualizing unstained specimens, and help the theoretical assurance with utilized content material and examples on designing structures and examining ends up in bio- and nanoscience applications.
- Provides a finished review of the foundations and methods of optical part microscopy and nanoscopy with biomedical applications.
- Tips/advice on development platforms and dealing with complicated imaging biomedical thoughts, together with interpretation of section photos, and methods for quantitative research in line with part microscopy.
- Interdisciplinary method that mixes optical engineering, nanotechnology, biology and scientific elements of this subject. each one chapter includes useful implementations and labored examples.
Read or Download Biomedical Optical Phase Microscopy and Nanoscopy PDF
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Additional info for Biomedical Optical Phase Microscopy and Nanoscopy
Iwasaki, J. Iwasaki, Differential interference contrast microscope with differential detection for optimizing image contrast, Appl. Opt. 35 (1996) 2230.  P. Hariharan, M. Roy, Achromatic phase-shifting for two-wavelength phase-stepping interferometry, Opt. Commun. 126 (1996) 220. R. G. R. I. J. Cogswell, Linear phase imaging using differential interference contrast microscopy, J. Microsc. 214(Pt 1) (2004) 7. V. R. Libertun, C. J. Cogswell, Calibration of a phase-shifting DIC microscope for quantitative phase imaging, Proc.
Lastly, it is of great importance to properly align the microscope illumination pathway for Ko¨hler illumination to ensure even illumination of the field. Image acquisition parameters set for high gain and contrast stretch accentuate even subtle variations in illumination intensity across the field of view. 2 Temperature Control Live cell imaging of mammalian somatic cells requires precise 37 C temperature control of the specimen with little temperature fluctuations. If the temperature rises too high, even transiently, the cells become stressed and viability is compromised.
Some processes may not be linear with temperature. For example, shifting HeLa cells from 37 to 33 C doubles their generation time and further reductions in temperature can differentially affect the kinetics of different cell cycle stages [2,3]. There are a number of ways to control the specimen temperature, but here we discuss the strategy we have found to be effective, safe, and reliable. The web pages listed at the end of this chapter provide a few sources of information that lay out other control strategies.