By David M. Langenau
This quantity makes a speciality of defining the original attributes of utilizing the zebrafish melanoma version for locating vital pathways and power drug goals for the therapy of human cancers. utilizing the zebrafish version, the amount explores oncogene and tumor suppressor discovery, chemical genetic methods, genomics, epigenetics, melanoma imaging, and telephone transplantation. Contributed chapters come from the main in demand laboratories operating during this box, which supplies a special point of view on zebrafish types from a large spectrum of the examine group. furthermore, the publication bargains a close research of the most up-tp-date study within the zone for particular zebrafish melanoma versions, together with T cellphone leukemia, rhabdomyosarcoma, liver and pancreatic melanoma, cancer, neuroblastoma, germ mobile tumors, and malignant peripheral sheath tumors. A bankruptcy is usually devoted to the advance and usage of different piscine types of melanoma. The compilation of chapters within the quantity culminates right into a finished and definitive textual content on zebrafish and melanoma, delivering a far wanted source at the robust attributes of the zebrafish version system.
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Extra info for Cancer and Zebrafish: Mechanisms, Techniques, and Models
Thus, for transgenesis only the gRNA needs to be designed. In zebraﬁsh the CRISPR/Cas9 system is applied by injection of a gRNA and the Cas9 mRNA from Streptococcus pyogenes into one-cell stage embryos and reveals high efﬁciency for knock-out of single genes (70–90 % [117–119]), as well as for simultaneous knock-out of multiple genes which allows analysis of both single and combinatorial knock-out effects already in the F0 generation. Through injection of two different gRNAs and Cas9 mRNA even huge chromosomal deletions are possible .
Cold Spring Harb Protoc 2013 69. van Lohuizen M, Verbeek S, Scheijen B, Wientjens E, van der Gulden H, Berns A (1991) Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging. Cell 65:737–752 70. Zender L, Xue W, Zuber J, Semighini CP, Krasnitz A, Ma B, Zender P, Kubicka S, Luk JM, Schirmacher P et al (2008) An oncogenomics-based in vivo RNAi screen identifies tumor suppressors in liver cancer. Cell 135:852–864 71. Lin WM, Baker AC, Beroukhim R, Winckler W, Feng W, Marmion JM, Laine E, Greulich H, Tseng H, Gates C et al (2008) Modeling genomic diversity and tumor dependency in malignant melanoma.
Even though they have a similar design to the lox sites, these frt sites are inert to Cre, which allows simultaneous application of both systems in a line, thus enhancing transgenic ﬂexibility. As the best activity of Flp is achieved at around 30 °C, an enhanced version (FLPe) was developed which retains its activity at higher temperatures, useful for applications in mammals . The Flp/frt system has been intensively used over a long time in drosophila, where it is an established tool (reviewed in [72, 73]) and in the mouse both alone [74, 75] and in combination with the Cre/lox system [76, 77].